DICOM PS3.17 2019b - Explanatory Information

YYY Preclinical Small Animal Imaging Acquisition Context (Informative)

YYY.1 

This Annex describes the use of Preclinical Small Animal Imaging Acquisition Context.

YYY.1.1 Example of housing and anesthesia for PET-CT

This Section contains examples for use cases involving imaging of a single animal in a hybrid PET-CT system.

The basic use case involves an animal, which:

  • lives in an individually ventilated home cage with several other animals in the same cage

  • is (briefly) transported (in its home cage) with its cage mates to the imaging facility, without heating, with an appropriate lid

  • is removed from its home/transport cage for preparation for imaging, involving insertion of a tail vein cannula, performed on an electrically heated pad

  • is induced by (a) placement in an induction chamber with more concentrated volatile anesthetic, or (b) intraperitoneal injection of Ketamine mixture

  • is placed in a PET-CT compatible imaging sled/carrier/chamber for imaging (of one animal at a time), with anesthesia with Isoflurane and Oxygen as the carrier gas, and heated with an electric pad regulated by feedback from a rectal probe

  • is removed for recovery in a separate cage

The content tree structure (when induction is by a volatile anesthetic) would resemble:

Node

Code Meaning of Concept Name

Code Meaning or Example Value

TID

1

Preclinical Small Animal Imaging Acquisition Context

TID 8101

1.1

Language of Content Item and Descendants

English

TID 1204

1.1.1

Country of Language

United States

TID 1204

1.2

Person Observer Name

Doe^Jane

TID 1003

1.3

Procedure Code

PET/CT FDG imaging of whole body

TID 1005

CID 100

1.4

Biosafety conditions

TID 8110

1.4.1

Biosafety level

Biosafety level 1

TID 8110

CID 601

1.5

Animal handling during specified phase

TID 8101

1.5.1

Phase of animal handling

In home cage

TID 8101

CID 634

1.5.2

Animal housing

TID 8121

1.5.2.1

Housing manufacturer

Acme Inc.

TID 8121

1.5.2.2

Housing rack product name

Acmerack IVC Mouse

TID 8121

1.5.2.3

Housing unit product name

Acmecage Mouse Pre-Bedded Corn Cob with Enrichment

TID 8121

1.5.2.4

Housing unit product code

12345

TID 8121

1.5.2.5

Housing unit lid product name

Acmecage IVC Mouse Single Filter

TID 8121

1.5.2.6

Housing unit lid product code

6789

TID 8121

1.5.2.7

Number of racks per room

4 {racks}

TID 8121

1.5.2.8

Number of housing units per rack

154 {housing units}

TID 8121

1.5.2.9

Housing unit location in rack

Row 4 Column 7

TID 8121

1.5.2.10

Number of animals within same housing unit

5 {animals}

TID 8121

1.5.2.11

Sex of animals within same housing unit

Female

TID 8121

1.5.2.12

Sex of handler

Mixed sex

TID 8121

1.5.2.13

Total duration in housing

133 days

TID 8121

1.5.2.14

Housing change interval

7 days

TID 8121

1.5.2.15

Manual handling interval

24 hours

TID 8121

1.5.2.16

Housing unit width

23.4 cm

TID 8121

1.5.2.17

Housing unit height

14.0 cm

TID 8121

1.5.2.18

Housing unit length

37.3 cm

TID 8121

1.5.2.19

Housing individually ventilated

Yes

TID 8121

CID 231

1.5.2.20

Air changes

50 /hour

TID 8121

1.5.2.21

Environmental temperature

22 C

TID 8121

1.5.2.22

Housing humidity

50 %

TID 8121

1.5.2.23

Housing unit reuse

Unused

TID 8121

CID 604

1.5.2.24

Bedding material

Corn cob bedding

TID 8121

CID 605

1.5.2.25

Bedding volume

450 ml

TID 8121

1.5.2.26

Enrichment material

Acmerichment paper twists

TID 8121

1.5.2.27

Exerciser device

Acmewheel

TID 8121

1.5.2.28

Shelter type

Red translucent igloo

TID 8121

CID 606

1.5.2.29

Shelter manufacturer

Acme Inc.

TID 8121

CID 606

1.5.2.30

Shelter product name

Acmedome

TID 8121

CID 606

1.6

Animal handling during specified phase

TID 8101

1.6.1

Phase of animal handling

During transport

TID 8101

CID 634

1.6.2

Animal housing

TID 8121

1.6.2.1

Housing manufacturer

Acme Inc.

TID 8121

1.6.2.2

Housing unit product name

Acmecage Mouse Pre-Bedded Corn Cob with Enrichment

TID 8121

1.6.2.3

Housing unit product code

12345

TID 8121

1.6.2.4

Housing unit lid product name

Acmecage Mouse Transport

TID 8121

1.6.2.5

Housing unit lid product code

9872

TID 8121

1.6.2.6

Number of animals within same housing unit

5 {animals}

TID 8121

1.6.2.7

Sex of animals within same housing unit

Female

TID 8121

1.6.3

Heating conditions

TID 8140

1.6.3.1

Heating

Unheated

TID 8140

CID 635

1.7

Animal handling during specified phase

TID 8101

1.7.1

Phase of animal handling

Staging prior to imaging

TID 8101

CID 634

1.8

Animal handling during specified phase

TID 8101

1.8.1

Phase of animal handling

Preparation for imaging

TID 8101

CID 634

1.8.2

Animal housing

TID 8121

1.8.2.1

Comment

Animal exposed and restrained whilst cannulating tail vein

TID 8121

1.8.3

Heating conditions

TID 8140

1.8.3.1

Heating

Electric heating pad

TID 8140

CID 635

1.8.3.2

Feedback temperature regulation

No

TID 8140

CID 231

1.9

Animal handling during specified phase

TID 8101

1.9.1

Phase of animal handling

Anesthesia induction

TID 8101

CID 634

1.9.2

Animal housing

TID 8121

1.9.2.1

Housing manufacturer

Acme Inc

TID 8121

1.9.2.2

Housing unit product name

Gas Anesthesia Induction Chamber Mouse

TID 8121

1.9.2.3

Housing unit product code

3487236

TID 8121

1.10

Animal handling during specified phase

TID 8101

1.10.1

Phase of animal handling

Imaging procedure

TID 8101

CID 634

1.10.2

DateTime Started

yyyymmddhhss

TID 8101

1.10.3

DateTime Ended

yyyymmddhhss

TID 8101

1.10.4

Animal housing

TID 8121

1.10.4.1

Housing manufacturer

Acme Inc

TID 8121

1.10.4.2

Housing unit product name

Multimodal Mouse Chamber

TID 8121

1.10.5

Heating conditions

TID 8140

1.10.5.1

Heating

Electric heating pad

TID 8140

CID 635

1.10.5.1

Feedback temperature regulation

Yes

TID 8140

CID 231

1.10.5.2

Temperature sensor device component

Rectal temperature

TID 8140

CID 636

1.10.5.3

Equipment Temperature

37 C

TID 8140

1.10.6

Physiological monitoring

TID 8170

1.10.6.1

Electrocardiographic monitoring

Yes

TID 8170

CID 231

1.10.6.2

Monitoring of respiration

No

TID 8170

CID 231

1.11

Animal handling during specified phase

TID 8101

1.11.1

Phase of animal handling

Anesthesia recovery period

TID 8101

CID 634

1.12

Administration of anesthesia

TID 8130

1.12.1

Anesthesia Method Set

TID 8130

1.12.1.1

Anesthesia Method

TID 8130

1.12.1.1.1

Anesthesia Category

General anesthesia

TID 8130

CID 611

1.12.1.1.2

Anesthesia Start Time

yyyymmddhhss

TID 8130

1.12.1.1.3

Anesthesia Finish Time

yyyymmddhhss

TID 8130

1.12.1.1.4

Anesthesia Induction

By inhalation

TID 8130

CID 613

1.12.1.1.5

Anesthesia Maintenance

Inhalation anesthesia system closed no rebreathing primary agent

TID 8130

CID 615

1.12.2

Airway Management Set

TID 8130

1.12.2.1

Airway Management

TID 8130

1.12.2.1.1

Airway Management Method

Nose cone

TID 8130

CID 617

1.12.2.1.2

Airway Sub-Management Method

Continuous flow ventilation

TID 8130

CID 619

1.12.3

Medications Set

TID 8130

1.12.3.1

Procedure Phase

During procedure

TID 8130

CID 631

1.12.3.2

Medication given

TID 8131

1.12.3.2.1

Drug start

yyyymmddhhss

TID 8131

1.12.3.2.2

Drug end

yyyymmddhhss

TID 8131

1.12.3.2.3

Route of administration

By inhalation

TID 8131

CID 11

1.12.3.2.4

Mixture

TID 8131

1.12.3.2.4.1

Drug administered

Isoflurane

TID 8131

CID 623

1.12.3.2.4.2

Medication Type

General anesthetic

TID 8131

CID 621

1.12.3.2.4.3

Concentration

4 %

TID 8131

1.12.3.2.5

Mixture

TID 8131

1.12.3.2.5.1

Drug administered

Oxygen gas

TID 8131

CID 623

1.12.3.2.5.2

Medication Type

Carrier gas

TID 8131

CID 621

1.12.3.2.5.3

Concentration

100 %

TID 8131

1.12.3.3

Medication given

TID 8131

1.12.3.3.1

Drug start

yyyymmddhhss

TID 8131

1.12.3.3.2

Drug end

yyyymmddhhss

TID 8131

1.12.3.3.3

Route of administration

By inhalation

TID 8131

CID 11

1.12.3.3.4

Mixture

TID 8131

1.12.3.3.4.1

Drug administered

Isoflurane

TID 8131

CID 623

1.12.3.3.4.2

Medication Type

General anesthetic

TID 8131

CID 621

1.12.3.3.4.3

Concentration

2 %

TID 8131

1.12.3.3.5

Mixture

TID 8131

1.12.3.3.5.1

Drug administered

Oxygen gas

TID 8131

CID 623

1.12.3.3.5.2

Medication Type

Carrier gas

TID 8131

CID 621

1.12.3.3.5.3

Concentration

100 %

TID 8131

The content tree structure when induction is by intra-peritoneal injection might be different in the following way, in that the housing during the induction phase does not involve a chamber, and the injected agent is specified, as follows:

Node

Code Meaning of Concept Name

Code Meaning or Example Value

TID

...

...

...

...

1.9

Animal handling during specified phase

TID 8101

1.9.1

Phase of animal handling

Anesthesia induction

TID 8101

CID 634

1.9.2

Animal housing

TID 8121

1.9.2.1

Comment

Animal exposed whilst inducing anesthesia

TID 8121

...

...

...

...

1.12

Administration of anesthesia

TID 8130

1.12.1

Anesthesia Method Set

TID 8130

1.12.1.1

Anesthesia Method

TID 8130

1.12.1.1.1

Anesthesia Category

General anesthesia

TID 8130

CID 611

1.12.1.1.2

Anesthesia Start Time

yyyymmddhhss

TID 8130

1.12.1.1.3

Anesthesia Finish Time

yyyymmddhhss

TID 8130

1.12.1.1.4

Anesthesia Induction

Intraperitoneal route

TID 8130

CID 613

1.12.1.1.5

Anesthesia Maintenance

Inhalation anesthesia, machine system, closed, no rebreathing of primary agent

TID 8130

CID 615

...

...

...

...

1.12.3

Medications Set

TID 8130

1.12.3.1

Procedure Phase

During procedure

TID 8130

CID 631

1.12.3.2

Medication given

TID 8131

1.12.3.2.1

Drug start

yyyymmddhhss

TID 8131

1.12.3.2.2

Route of administration

Intraperitoneal route

TID 8131

CID 11

1.12.3.2.3

Mixture

TID 8131

1.12.3.2.3.1

Drug administered

Ketamine

TID 8131

CID 623

1.12.3.2.3.2

Medication Type

General anesthetic

TID 8131

CID 621

1.12.3.2.3.2

Dosage

nn mg

TID 8131

1.12.3.2.4

Mixture

TID 8131

1.12.3.2.4.1

Drug administered

Medetomidine

TID 8131

CID 623

1.12.3.2.4.2

Medication Type

General anesthetic

TID 8131

CID 621

1.12.3.2.4.2

Dosage

nn mg

TID 8131

...

...

...

...

YYY.1.2 Example of exogenous substance administration to encode tumor cell line

Only the exogenous substance information is included in this example and content describing animal handling, anesthesia information, etc. is excluded for clarity. Indeed, given the optionality of the other content, it would be possible to create an Acquisition Context SR instance that describes only the exogenous substance information and nothing else.

The content tree structure would resemble:

Node

Code Meaning of Concept Name

Code Meaning or Example Value

TID

1

Preclinical Small Animal Imaging Acquisition Context

TID 8101

1.1

Language of Content Item and Descendants

English

TID 1204

1.1.2

Country of Language

United States

TID 1204

1.2

Person Observer Name

Doe^Jane

TID 1003

... ... ... ...

1.n

Exogenous substance

TID 8182

1.n.1

Tumor Graft

Adenocarcinoma

TID 8182

CID 637

CID 639

1.n.1.1

Age Started

6 week

TID 8182

CID 7456

1.n.1.2

DateTime Started

yyyymmddhhss

TID 8182

1.n.1.3

Brand Name

MDA-MB-468

TID 8182

1.n.1.4

Dosage

10E6 {cells}

TID 8182

CID 6092

1.n.1.5

Relative dose frequency

Single event

TID 8182

CID 6094

CID 6091

1.n.1.6

Route of Administration

Subcutaneous route

TID 8182

CID 11

1.n.1.6.1

Site of

Flank

TID 8182

CID 644

1.n.1.6.1.1

Laterality

Left

TID 8182

CID 244

1.n.1.7

Tissue of origin

Breast

TID 8182

CID 645

1.n.1.8

Taxonomic rank of origin

homo sapiens

TID 8182

CID 7454

YYY.1.3 Informative References

YYY.1.3.1 Method Descriptions

[Stout et al 2013] Molecular Imaging. Stout D, Berr SS, LeBlanc A, Kalen JD, Osborne D, Price J, Schiffer W, Kuntner C, and Wall J. 2013. 12. 7. 1-15. “Guidance for Methods Descriptions Used in Preclinical Imaging Papers”. http://journals.sagepub.com/doi/pdf/10.2310/7290.2013.00055 .

[David et al 2013a] Comparative Medicine. David JM, Chatziioannou AF, Taschereau R, Wang H, and Stout DB. 2013. 63. 5. 386–91. “The Hidden Cost of Housing Practices: Using Noninvasive Imaging to Quantify the Metabolic Demands of Chronic Cold Stress of Laboratory Mice”. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796748/ .

[David et al 2013b] Journal of the American Association for Laboratory Animal Science. David JM, Knowles S, Lamkin DM, and Stout DB. 2013. 52. 6. 738–44. “Individually Ventilated Cages Impose Cold Stress on Laboratory Mice: A Source of Systemic Experimental Variability”. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838608/ .

[Rosenbaum et al 2009] Journal of the American Association for Laboratory Animal Science. Rosenbaum MD. 2009. 48. 6. 763–73. “Effects of Cage-Change Frequency and Bedding Volume on Mice and Their Microenvironment”. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2786931/ .

[Fueger et al 2006] Journal of Nuclear Medicine. Fueger BJ. 2006. 47. 6. 999–1006. “Impact of Animal Handling on the Results of 18F-FDG PET Studies in Mice”. http://jnm.snmjournals.org/content/47/6/999 .

[Dandekar et al 2007] Journal of Nuclear Medicine. Dandekar M. 2007. 48. 4. 602–7. “Reproducibility of 18F-FDG microPET Studies in Mouse Tumor Xenografts”. doi:10.2967/jnumed.106.036608 http://jnm.snmjournals.org/content/48/4/602 .

[Lee et al 2005] Journal of Nuclear Medicine. Lee KH. 2005. 46. 9. 1531–36. “Effects of Anesthetic Agents and Fasting Duration on 18F-FDG Biodistribution and Insulin Levels in Tumor-Bearing Mice”. http://jnm.snmjournals.org/content/46/9/1531 .

[Balcombe et al 2004] Journal of the American Association for Laboratory Animal Science. Balcombe JP. 2004. 43. 6. 42–51. “Laboratory Routines Cause Animal Stress”. .

[Van der Meer et al 2004] Journal of the American Association for Laboratory Animal Science. Van der Meer E. 2004. 38. 4. 376–83. “Short-term effects of a disturbed light–dark cycle and environmental enrichment on aggression and stress-related parameters in male mice”. http://www.animalexperiments.info/resources/Studies/Animal-impacts/Stress.-Balcombe-et-al-2004./Stress-Balcombe-et-al-2004.pdf .

[Tabata et al 1998] Laboratory Animals. Tabata H. 1998. 32. 2. 143–48. “Comparison of Effects of Restraint, Cage Transportation, Anaesthesia and Repeated Bleeding on Plasma Glucose Levels between Mice and Rats”. doi:10.1258/002367798780599983 http://lan.sagepub.com/content/32/2/143 .

DICOM PS3.17 2019b - Explanatory Information